The momentum of artificial intelligence (AI) investment in the United States has continued its strong trajectory into 2025, building on the significant growth from the previous year. According to TechCrunch, 2024 saw 49 startups secure funding rounds of $100 million or more, with three companies completing multiple mega-rounds and seven raising $1 billion or more. As 2025 enters its final quarter, this trend shows no signs of slowing down, with multiple billion-dollar rounds already announced and several firms receiving repeat mega-round investments.
A Chronological List of US-Based AI Startups Raising $100M+ in 2025
August
- EliseAI: A healthcare and housing automation platform, secured $250 million in a Series E round led by Andreessen Horowitz, reaching a valuation of $2.2 billion.
- Decart: An AI research laboratory, raised $100 million at a $3.1 billion valuation. The round included Sequoia Capital, Benchmark, and Zeev Ventures.
July
- Fal: A generative media platform, completed a $125 million Series C round led by Meritech Capital Partners, valued at $1.5 billion.
- Ambience Healthcare: The developer of an AI healthcare operating system, raised $243 million in a Series C round led by Oak HC/FT and Andreessen Horowitz.
- Reka AI: Secured $110 million in a Series B round with investors including Snowflake and Nvidia, valuing the company at $1 billion.
- Thinking Machines Lab: An AI research firm, raised a staggering $2 billion in a seed round led by Andreessen Horowitz, with a valuation of $12 billion.
- OpenEvidence: Based in Cambridge, Massachusetts, raised $210 million at a $3.5 billion valuation in a Series B round led by Kleiner Perkins and GV.
- Harmonic: Focused on mathematical reasoning, raised $100 million in a Series B round led by Kleiner Perkins, with a valuation of $875 million.
June
- Abridge: A healthcare AI unicorn, raised $300 million in a Series E round led by Andreessen Horowitz, valuing the company at $5.3 billion.
- Harvey: An AI provider for legal services, raised its second $300 million round of 2025 in a Series E round co-led by Kleiner Perkins and Coatue, with a valuation of $5 billion.
- Tennr: A healthcare AI startup, completed a $101 million Series C round led by IVP, with a valuation of $605 million.
- Glean: An enterprise search startup, raised $150 million in a Series F round led by Wellington Management, and is now valued at $7.25 billion.
- Anysphere: The AI research company behind Cursor, raised $900 million in a Series C round led by Thrive Capital, with a valuation of nearly $10 billion.
May
- Snorkel AI: A data labeling startup, raised $100 million in a Series D round led by Addition, reaching a $1.3 billion valuation.
- LMArena: A benchmarking platform for AI models, completed a $100 million seed round co-led by Andreessen Horowitz and UC Investments, with a valuation of $600 million.
- TensorWave: Based in Las Vegas, raised $100 million in a Series A round co-led by Magnetar Capital and AMD Ventures.
April
- SandboxAQ: An AI model firm, completed a $450 million Series E round, valuing the company at $5.7 billion. Investors included Nvidia, Google, and Ray Dalio.
- Runway: An AI company specializing in media production, raised $308 million in a Series D round led by General Atlantic, with a valuation of $3 billion.
March
- OpenAI: Raised a record-breaking $40 billion in funding, resulting in a valuation of $300 billion. The round was led by SoftBank.
- Nexthop AI: Raised $110 million in a Series A round led by Lightspeed Venture Partners.
- Insilico Medicine: Based in Cambridge, Massachusetts, raised $110 million in a Series E round co-led by Value Partners and Pudong Chuangtou, with a valuation of $1 billion.
- Celestial AI: An infrastructure firm, secured $250 million in a Series C round led by Fidelity, reaching a $2.5 billion valuation.
- Lila Sciences: Raised $200 million in a seed round to develop a science superintelligence platform, led by Flagship Pioneering.
- Reflection.Ai: Based in Brooklyn, raised $130 million in a Series A round led by Lightspeed, with a valuation of $580 million.
- Turing: An AI coding firm, raised $111 million in a Series E round led by Khazanah Nasional, with a valuation of $2.2 billion.
- Shield AI: A defense-focused AI startup, secured $240 million in a Series F round co-led by L3Harris Technologies and Hanwha Aerospace, valued at $5.3 billion.
- Anthropic: A leading AI research company, completed a $3.5 billion Series E round led by Lightspeed, with a valuation reaching $61.5 billion.
February
- Together AI: Raised $305 million in a Series B round co-led by Prosperity7 and General Catalyst, with a valuation of $3.3 billion.
- Lambda: An infrastructure company, secured $480 million in a Series D round led by SGW and Andra Capital, with a valuation of nearly $2.5 billion.
- Abridge: Announced a separate $250 million Series D round led by IVP and Elad Gil, with a valuation of $2.75 billion.
- Eudia: A legal tech company, raised $105 million in a Series A round led by General Catalyst.
- EnCharge AI: Raised $100 million in a Series B round led by Tiger Global.
- Harvey: In an earlier round this year, raised $300 million in a Series D round led by Sequoia, with a valuation of $3 billion.
January
- ElevenLabs: A synthetic voice startup, raised $180 million in a Series C round co-led by ICONIQ Growth and Andreessen Horowitz, with a valuation of over $3 billion.
- Hippocratic AI: Which develops large language models for the healthcare sector, raised $141 million in a Series B round led by Kleiner Perkins, with a valuation of over $1.6 billion.
